| Steriliser steam penetration test (Helix or Bowie-Dick), vacuum sterilisers only, and automatic control test, with the temperature and pressure recorded | Daily, before the steriliser is used for instruments | User or, by delegation, operator | England: HTM 01-05 paragraphs 4.16 to 4.21 and 12.5 |
| Steriliser air leakage test (type B and S) and residual air test (type S and N) | Weekly, with the daily tests | User or, by delegation, operator | England: HTM 01-05 paragraph 12.5 |
| Steriliser thermometric tests | Quarterly, or as the manufacturer recommends | Competent Person (Decontamination) or service engineer | England: HTM 01-05 paragraph 12.5 |
| Steriliser validation, with the annual thermometric, dryness and steam generator overheat cut-out tests | At installation, annually and after major repairs | Competent Person (Decontamination) or service engineer | England: HTM 01-05 paragraphs 12.2 and 12.5 |
| Washer-disinfector: strainers and filters cleaned, and every load examined | Daily | User or, by delegation, operator | England: HTM 01-05 paragraph 13.3 |
| Washer-disinfector protein residue test and door seal check | Weekly | User or, by delegation, operator | England: HTM 01-05 paragraph 13.3 |
| Washer-disinfector door interlocks, automatic control, cleaning efficacy, chemical dosing and thermometric disinfection tests | Quarterly, or as the manufacturer recommends; all validation tests annually | Competent Person (Decontamination) or service engineer | England: HTM 01-05 paragraphs 13.2 and 13.3 |
| Ultrasonic cleaner: strainers and filters cleaned, drained at the end of the day or session, and every load examined | Daily | User or, by delegation, operator | England: HTM 01-05 paragraph 14.3 |
| Ultrasonic cleaner door seal check and protein residue test | Weekly | User or, by delegation, operator | England: HTM 01-05 paragraph 14.3 |
| Ultrasonic cleaner automatic control, calibration, cleaning efficacy and ultrasonic activity tests | Quarterly, or as the manufacturer recommends; all validation tests annually | Competent Person (Decontamination) or service engineer | England: HTM 01-05 paragraphs 3.29 and 14.3 |
| Dental unit waterlines flushed | At least two minutes at the start and end of the day and after a significant period unused, such as lunch; at least 20 to 30 seconds between patients | Practice staff | England: HTM 01-05 paragraph 6.85 |
| Decontamination audit | At least every six months | The practice, with the IPS/DH audit tool strongly recommended | England: HTM 01-05 paragraphs 1.7 and 2.21 |
| Spore test (biological indicator) on each steriliser | At least weekly, with mechanical and chemical monitoring | Practice staff | United States: CDC, Sterilization and Disinfection |
| Dental unit water quality monitoring, to 500 CFU/mL or fewer of heterotrophic water bacteria | Routinely, as the manufacturer of the unit or waterline treatment product recommends | Practice staff | United States: CDC, Dental Unit Water Quality |